Study on Orthogonal Experiment and Affecting Factors on Pb

Abstract Dynamic adsorption of dead-immobilized Saccharomyces cerevisiae on Pb in continuous-upflow reactor was studied through orthogonal experiment to investigate the optimal operating parameters of pH value、flow rate、adsorbent concentration as well as affect factors, such as yeast diameter、initial Pb concentration and initial pH value. It showed that the optimal conditions were of pH6、flow rate 5mL/min、adsorbent concentration 80 g/L. And the optimal diameter was 2.5mm, initial Pb concentration 20mg/L, optimal pH value 6. In the early 40 minutes of the experiment, Ct/C increased fast, which indicated that a majority of, up to 92% of Pb was adsorbed. The adsorption process by dead-immobilized discarded Saccharomyces cerevisiae in continuous-upflow reactor was very fast. It was feasible to wipe off Pb in wastewater by continuous-upflow reactor, which could guide future researchers and establish foundation for its industrialization. Authors Brief Zhang Ying, female, professor. Research in bioremediation on pollutants. Corresponding author: Fan Chunhui, (1982), male, doctor. Research in waste treatment and reutilization.